【摘要】 对23头圈养黑熊进行63次电刺激采精,并在对精液进行品质检查的基础上,通过对3种稀释液4℃保存精液结果的筛选比较试验,得出以下结果:圈养黑熊采精量平均为1.06±0.93ml,pH值为6.71±0.44,精子密度为(3.23±1.68)×108个/ml,精子畸形率为(23.48±7.95)%,顶体完整率为(88.58±3.86)%,精子活率和活力分别为(76.30±13.91)%和(63.91±18.53)%;Tris-柠-果-葡-卵稀释液保存圈养黑熊精液效果最好,可作为进一步研究优化的首选。更多还原

【Abstract】 On the basis of the bear semen collected by electroejaculation for 63 times from 23 captive black bears, the semen trait was determined. Through screening comparison of 3 dilutions of semen preserved under the 4℃ temperature, the results showed: the average of ejaculation of captive black bears was 1.06±0.93 ml, pH.6.71±0.44, sperm count (3.23±1.68)×10 8/ml, abnormal sperm percentage (23.48±7.95)%, intact acrosome percentage (88.58±3.86)%, motility and vitality (76.30±13.91)% and (63.91±18.53)%. The effect of Tris-citric acid-fructose-glucose-yelk dilution preserved the captive black bear semen were the best. The dilution of Tris-citric acid-fructose-glucose-yelk can be optimized for further study as the first choice.更多还原