【摘要】 采用原位杂交技术研究地塞米松对大鼠再生肝细胞亚精胺合成酶(spermidine syathase,SPDS)基因表达的影响。结果显示,部分肝切除(partial hepatectomy,PH)后,再生肝细胞mRNA表达量呈现先升高后降低的变化趋势,峰值均出现在PH后9h。PH+去肾上腺使mRNA水平升高,主要表现在PH后6～12h;地塞米松处理组SPDS基因表达量明显下降,并且随着地塞米松剂量升高,mRNA表达水平降低。结果表明,地塞米松对早期再生肝细胞SPDS基因表达具有抑制作用。更多还原

【Abstract】 The effect of dexamethasone on gene expression of spermidine synthase(SPDS) in regenerating rat hepatocytes was investigated by in situ hybridization.The results showed:in all partial hepatectomy(PH) rats,expressing level of SPDS was gradually rising,then falling with time,and the highest was at 9th h after PH.In the adrenalectomized rats with PH,SPDS mRNA level rose after PH,especially from 6th to 12th h.However,the adrenalectomized rats with PH exposured to examethasone(0.1,0.2,and 2 mg/kg),the expressing level of SPDS was reduced with dose-dependent manner.The results suggested that gene expression of SPDS was inhibited by dexamethasone in the regenerating rat hepatocytes,mainly in early stage of liver regeneration.更多还原