【摘要】 本文以外标法,6-氨基喹啉基-N-羟基琥珀酰亚氨基甲酸酯(AQC)为柱前衍生试剂,用AccQ.Tag氨基酸分析柱(3.9mm×150mm,4μm,美国),柱温37℃,缓冲盐、乙腈和水梯度洗脱,光电二极管(PDA)检测器在248nm波长处检测,测定了稻米中17种氨基酸的含量,取得了较理想的结果。该法简便、准确、可靠。更多还原

【Abstract】 This paper studied on determination of 17 kinds of amino acids content in rice by waters AccQ-Tag pre-column derivatization. The amino acids were derivatized with AccQ.FluorTM Reagent Kit.The chromatography condition was: Buffer- acetonitrile –water as mobile phase and grads wash and an AccQ.Tag Amino Acid Analysis Column (3.9mm ×150mm,4μm ,U.S.A) was used at 37℃,The photoelectric diode detector was operated at 248nm..The standard curve were determined for amino acids standard sample. The method has good accuracy and repeatability and dependability.更多还原