【摘要】 目的探讨重组腺病毒Ad-Rb94基因联合放射治疗对人肝癌细胞的联合抑瘤作用。方法将重组腺病毒Ad-Rb94基因导入人肝癌细胞株HepG2,观察联合放射治疗对细胞生长、细胞周期及凋亡的影响。结果Ad-Rb94基因组、放疗组和Ad-Rb94基因联合放疗组HepG2细胞的生长均受到抑制,尤其在转染后96h细胞存活数量最低,与Ad-lacZ组和空白对照组比较差异均有显著性(P<0.05)。联合治疗组HepG2细胞生长最为缓慢,Rb94基因转染后96hAd-Rb94联合放疗组的抑制作用均明显高于Ad-Rb94组和放疗组(P(0.05)。Ad-Rb94组、放疗组及Ad-Rb94联合放疗组HepG2停留在G2/M期的细胞增加,G0/G1期和S期细胞数量减少,凋亡细胞数量增加,其中以联合治疗组G2/M期细胞和凋亡细胞所占比例最高。结论Ad-Rb94基因联合放疗具有协同作用,有效地抑制肝癌细胞的生长。更多还原

【Abstract】 Objective To evaluate the effects of recombinant adenovirus-Rb94 and radiotherapy on the growth of human hepatic carcinoma cells.Methods Human hepatic carcinoma cells HepG2 were treated with Ad-Rb94,radiotherapy or Ad-Rb94 combined with radiotherapy respectively.The cell growth curve,cell cycle and cell apoptosis of HepG2 cells were examined.Rusults The growth of HepG2 cells transfected with Ad-Rb94,radiation and combination of Ad-Rb94 and radiation was significantly slower than that of Ad-lacZ and blank control(P<0.05),especially at 96h after transfection.The combination of Ad-Rb94 and radiation resulted in greater inhibition of cell growth compared with Ad-Rb94 infection and radiation at 96h after transfection(P<0.05).The number of cells of G2/M phases transfected with Ad-Rb94 and radiation increased,while the number cells of G0/G1 and S phases decreased;cells of apoptosis increased.The increase of cells of G2/M phases and apoptosis for the combination of Ad-Rb94 and radiation were the highest.Conclusion Ad-Rb94 gene therapy combined with radiotherapy show synergism for the inhibition of HepG2 cells growth.更多还原