【摘要】 目的观察低剂量辐射诱导EL-4淋巴瘤细胞周期进程的适应性反应。方法用X射线照射离体EL-4淋巴瘤细胞,其诱导剂量(D1)为25~200mGy(12.5mGy/min),攻击剂量(D2)为1.5Gy(287mGy/min),D1和D2间隔6h。通过流式细胞仪检测其细胞周期各时相百分数的变化。结果当D1和D2分别为25~100mGy和1.5Gy,或分别为75mGy和0.5~2.0Gy,D1+D2各组G0/G1期细胞百分数不同程度低于各自D2组,而S期细胞百分数明显高于各自D2组(P<0.05、P<0.01或P<0.001)。结论上述结果显示,EL-4淋巴瘤细胞在1.0~2.0Gy照射前6h接受25~100mGy照射,可在体外诱导其细胞周期进程的适应性反应。更多还原

【Abstract】 Objective To explore the adaptive response of EL-4 cycle progression induced by low dose radiation in vitro.Methods EL-4 lymphoma cells were irradiated with the inductive doses(D1:25-200 mGy,12.5 mGy/min)and the challenging dose(D2:0.5-3.0 Gy,287 mGy/min).The interval time between D1 and D2 was 6 h.The cell percentages of each cell cycle phase were measured with flow cytometry.Results The cell percentages of G0/G1 phase in the D1 + D2 group with25-100 mGy(D1)and 1.5 Gy(D2)or 75 mGy(D1)and 25-200 mGy(D2)were invaribly lower than those in the D2 group,while the cell percentages of S phase increased significantly(P<0.05,P<0.01 or P<0.001).Conclusion The results suggest that EL-4 lymphoma cells were irradiated with 25-100 mGy at 6h before 1.0-2.0 Gy exposure.The low-dose radiation could induce the adaptive response of the cell cycle progression in vitro.更多还原