【摘要】 以"巴西"香蕉为材料,在人工气候箱条件下,研究不同温度(16,19,22,25,28,31,34℃)及不同光照(1 000,1 500,3 000,5 000,8 000 lx)对香蕉组培苗增殖和生根的影响。结果表明,温度对香蕉分化芽的增殖和生根均有极显著影响;光照主要影响分化芽和生根苗的长势,而对香蕉分化芽的增殖和生根没有显著影响;温度与光照的互作对香蕉分化芽的增殖没有显著影响,而对分化芽的长势和生根均有显著的影响。31℃时香蕉分化芽的倍增率和生根率均达到最大,分别为3.07和0.98。增殖培养阶段香蕉分化芽的叶绿素含量低于生根培养阶段。当温度在25～31℃之间、光照在1 000～3 000 lx之间时,叶绿素含量均相对较高。高、低温及强光照下叶绿素含量均相对较低。更多还原

【Abstract】 ’Brazil’ banana widely cultivated in Guangdong was selected as material,and 7 temperature levels,16,19,22,25,28,31 and 34 ℃,and 5 light intensity levels,1 000,1 500,3 000,5 000 and 8 000 lx,were employed in the phytotrone to observe the growth and multiplication of banana plantlets in tissue culture in the laboratory of Guangdong Ocean University from 2004 to 2005.Temperature was found to have significant effects on bud multiplication and root formation in banana tissue culture,and light had significant effects on growth of buds and plantlets but little effects on bud multiplication and root formation.The interaction between temperature and light influences significantly the growth of buds and formation of root,but not significantly the multiplication of buds.The rates of multiplication and root formation were the highest at the temperature of 31 ℃,3.07 and 0.98,respectively,and the chlorophyll content in bud was relatively high at the stages of both multiplication and root formation when the temperature ranged from 25 ℃ to 31 ℃ and the light intensity from 1 000 lx to 3 000 lx.The content of bud chlorophyll was relatively low in extremely high or low temperatures,or under strong illumination during both bud multiplication and root formation.更多还原