节点文献
1株产纤维素细菌的初步鉴定
Isolation and Preliminary Identification of a Cellulose-producing Bacteria Strain
【摘要】 从果胶中分离得到1株产纤维素膜的菌株AXI,对其16SrRNA序列进行克隆和分析。结果表明,菌株AXI的16SrRNA序列与醋杆菌属菌种的同源性达99%,结合其形态和生理生化特征研究结果,将其归为醋杆菌属。
【Abstract】 The bacteria AXI that can produce cellulose membrane was isolated from pectin. The isolate was identified by ways of its morphological, physiological and biochemical characteristics. In addition, molecular identification of the 16SrRNA gene of the isolate was conducted. The 16SrRNA gene was sequenced, and molecular phylogenetic tree was constructed. Results indicated that the bacteria AXI isolated was most similar to Acetobacter. The 16SrRNA sequence of the AXI was 99 % homogenous to that of the Acetobacter. The comprehensive analysis showed that the AXI was grouped to Acetobacter.
【关键词】 分离;
分子鉴定;
16SrRNA;
醋杆菌属;
纤维素;
系统进化树;
【Key words】 isolation molecular identification 16SrRNA Acetobacter cellulose phylogenetic tree;
【Key words】 isolation molecular identification 16SrRNA Acetobacter cellulose phylogenetic tree;
【基金】 科技部“国家科技基础条件平台工作”(编号:2005DKA30506);国家科技部“科技基础工作和社会公益研究专项”(编号:2004DIB3J073)
- 【文献出处】 热带作物学报 ,Chinese Journal of Tropical Crops , 编辑部邮箱 ,2008年02期
- 【分类号】Q93
- 【被引频次】3
- 【下载频次】174