【摘要】 目的建立一种检测血清丙肝病毒抗体IgM的方法。方法将丙肝病毒抗原点于硝基纤维膜上,应用Φ15nm的羊抗人IgM金标抗体与血清标本中的丙肝病毒IgM结合,再加银加强试剂,阳性者为棕黑色。结果整个试验过程需要10m in,检测HCVAb/HCVRNA阳性临床标本,结果有良好的一致性,总符合率为96%。结论银强化滴金免疫法检测血清丙肝病毒抗体IgM,操作简单,试剂稳定、敏感、快速,适于基层。更多还原

【Abstract】 Objective To establish a more rapid and simple method for detecting anti-HCV IgM antiboy tin patient’s serum.Methods A silver enhanced dot immunogold filtration assay(SEDIGFA)was developed by coating the antigen protein of HCV on nitrocellulose(NC) membrance,adding colloidal gold labeled sheep anti-human IgM antibody and the silver enhancer.The whole procedure can be finished within 10 minutes.Results The coincident rate of serum samples positive for HCVAb/HCVRNA detected with this method was 96%.Conclusion SEDIGFA is more simple and it can be used for rapid detecttion of anti-HCV IgM in small medical laboratories.更多还原