【摘要】 目的观察免疫层析试纸条对实验感染猪肉汁中抗旋毛虫抗体的检测效果。方法以胶体金标SPA和旋毛虫肌幼虫ES抗原制备了免疫层析试纸条。将30头家猪随机分为2组:实验感染组20头,每头经口感染5000条旋毛虫幼虫,正常对照组10头。感染后10w用试纸条进行血清及肉汁抗体检测,并与镜检法进行比较。结果应用试纸条检测旋毛虫幼虫感染的家猪血清及肉汁的抗体阳性率均为100%(20/20),肌肉压片镜检法的幼虫检出率亦为100%(20/20),每克腿肌虫荷为111～400条幼虫(平均为231.36条)。应用试纸条和镜检法对正常对照组肉汁及膈肌时均为阴性。感染猪肌肉-20℃保存8个月的肉汁抗体阳性率亦为100%(20/20)。结论试纸条检测肉汁中旋毛虫抗体可用于新鲜肉及冷冻猪肉中旋毛虫检疫的初筛。更多还原

【Abstract】 Objective To observe the effect of the immunochromatographic strip for detection of anti-Trichinella antibodies in muscle juice of experimentally infected domestic pigs.Methods Immunochromato-graphic strip was prepared by using SPA labeled with colloidal gold and T.spiralis muscle larval excretory-se-cretory(ES) antigens.Thirty male Ningxiang pigs were randomly divided into 2 groups.The experimental group with 20 pigs and control group with 10 pigs.Each pig in the experimental group was orally inoculated with 5 000 motile muscle larvae of T.spiralis.The anti-Trichinella antibodies in serum and muscle juice were assayed by the strip 10 weeks after infection.The results of strip were compared with those of trichinelloscopy.Results The positive rate of anti-Trichinella antibody for the serum and muscle juice of the infected pigs was 100%(20/20),all the leg muscle samples were detected the larvae by trichinelloscopy with 111～400 larvae per gram(lpg)(mean 231.36).The muscle juice and diaphragm samples from the control group were also exam-ined by the strip and trichinelloscopy,respectively,the results were negative by both methods.The positive rate was 100%(20/20) for the infected pork conserved at -20℃ for 8 months.Conclusion The immunochro-matographic strip for detection of anti-Trichinella antibodies in muscle juice could be used for the preliminary screening on fresh and frozen pork.更多还原