【摘要】 目的观察胰腺癌组织淋巴管内皮细胞LYVE-1的表达,为研究癌淋巴管转移的分子机制提供形态学依据。方法用免疫组化的方法对人胰腺癌和正常胰腺组织中LYVE-1的表达情况进行观察,用LYVE-1来标记淋巴管,检测癌组织中微淋巴管密度(LMVD)。结果胰腺癌组织血管内皮细胞LYVE-1不染色,淋巴管内皮细胞LYVE-1阳性表达,经计数微淋巴管数量,癌组织中的LMVD明显高于癌旁正常胰腺组织(P<0.01),并且LMVD的表达与VEGF-C、VEGF-D的表达具有显著相关性(P<0.01)。结论LYVE-1可选择性的表达在胰腺癌组织淋巴管内皮上,能明确的区分血管及淋巴管成分,可作为一个淋巴管内皮细胞较特异的标记物。由于LMVD在癌组织中的表达明显增高,说明淋巴管数量的增加可能与胰腺癌的淋巴转移有关。更多还原

【Abstract】 Objective To provide the morphology data for the study on the molecular mechanism of tumor metastasis, we observed the expression of LYVE-1 on the lymphatics in the human pancreatic carcinoma. Methods We observed the expression of LYVE-1 by SP immunohistochemistry. LMVD in pancreatic carcinoma was calculated by using LYVE-1 as the specific marker of lymphatic endothelium. Results LYVE-1 has not been stained ,but LYVE-1 was positively expressed on the colonic carcinoma lymphatics.The expression positive rate of LMVD in pancreatic carcinoma was obviously higher than that in normal pancreatic tissues(P<0.01).The difference existed between the LMVD and VEGF-C、VEGF-D(P<0.01)in pancreatic carcinoma. Conclusions LYVE-1 choosed to expresse in lymphatics in the colonic carcinoma and could correctly distinguish the blood vessels and lymphatic vessels,could be regarded as the specific marker to mark lymphatic vessel.And, The LYVE-1 expression positive rate of the colonic carcinoma were increased in pancreatic carcinoma and the group with lymph nodes metastasis.It indicates,It was correlated with colonic carcinoma lymphatical metastasis that the count of Lymphatic vessels were increased.更多还原