【摘要】 目的:探讨诱导分化剂对K562细胞增殖抑制的作用与分子机制。方法:通过MTT试验和细胞形态学观察K562细胞增殖的改变和分化情况。通过流式细胞仪实验检测细胞周期改变,RT-PCR检测EVI1、TGF-β1和WT1基因表达的变化。结果:K562细胞经六亚甲基二乙酰胺(HMBA)和(或)三氧化二砷(As2O3)作用后增殖明显受到抑制,并向不同方向分化,细胞被阻滞在G1期。K562细胞的增殖抑制作用与EVI1和WT1基因表达下调,TGF-β1基因表达上调一致。EVI1/β-actin从0.9253渐降至0.1379,WT1/β-actin从0.9953降至0.1978。TGF-β1/β-actin从0.3315增至1.2452。结论:HMBA、As2O3及其联合抑制K562细胞增殖作用与EVI1和WT1基因表达下调,TGF-β1基因表达上调有关。更多还原

【Abstract】 OBJECTIVE: To analyze the inhibitory effect and the molecular mechanism of the induction differentiation on the multiplication of K562 cell. METHODS:The multiplication and differentiation of K562 cell were observed through the MTT experiment and the cytomorphology. The flow cytometer was used to examine the cell cycle changes, and the RT-PCR was used to test EVI1, TGF-β1 and WT1 gene expression changes. RUSULTS:The multiplication of was inhibitated K562 cell was inhibitated obviously was inhibitated by HMBA or/and As2O3. The cells were hindered in the G1 stage, which was consistent to EVI1 and the WT1 gene expression declines, and TGF-β1 gene expression upward. EVI1/β-actin fell deereased from 0.925 3 to 0.137 9, and WT1/β-actin fell from 0.995 3 to 0.197 8. TGF-β1/β-actin increased from 0.331 5 to 1.245 2. CONCLUSION:HMBA or/and As2O3 suppresse the K562 cell multiplication function which is related to EVI1 and the WT1 gene expression declines and TGF-β1 gene expression upward.更多还原