【摘要】 目的:探讨中国北方胃癌高、低发区人群HLA-DPB1等位基因多态性分布与胃疾病及幽门螺杆菌(Helicobacter pylori,Hp)感染的关系。方法:采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)技术分析863例研究对象的HLA-DPB1等位基因多态性;酶联免疫吸附方法(ELISA)检测血清Hp-IgG。结果:1)中国北方地区人群中检出4种DPB1等位基因多态:DPB1*1401(65%)最高,其次依次为*1701(5.4%)、*0901(4.0%)和*0301(0.9%)。2)胃癌高、低发人群DPB1*0901和*1701等位基因频率分布差异有统计学意义(*0901:χ2=36.145,P<0.01;*1701:χ2=35.526,P<0.01)。3)胃癌与非癌对照组间DPB1*0901、*1401、*1701等位基因频率分布差异无统计学意义;Hp是否感染组间DPB1*0901、*1401、*1701等位基因频率分布差异无统计学意义。结论:HLA-DPB1*0901、*1701等位基因多态存在胃癌高、低发区人群分布差异,未发现HLA-DPB1等位基因多态与胃癌易感性及Hp感染有关。更多还原

【Abstract】 OBJECTIVE:To investigate the relationship between HLA-DPB1 polymorphisms and gastric cancer and the infection of Hp in population at the high and low areas of gastric carcinoma in northern China.METHODS: PCR-RFLP was applied to analysis HLA-DPB1 polymorphisms in 863 cases and ELISA was used to determine Hp-IgG.RESULTS: 1) Four HLA-DPB1 polymorphisms were detected: The most predominant DPB1 allele was DPB1*1401 with the frequency of(65%),followed by DPB1*1701(5.4%),*0901(4.0%),*0301(0.9%);2) *0901 and *1701 alleles were found significantly different in the poqulation between the high and low areas of gastric carcinoma(*0901: χ2=36.145,P<0.01;1701:χ2=35.526,P<0.01);3)Between the no gastric cases and gastric carcinoma,no difference among DPB1*0901,*1401 and *1701 was found;no significant difference was found among DPB1*0901,*1401,*1701 alleles between Hp+positive and Hp-negative population.CONCLUSIONS: HLA-DPB1 *0901 and *1701 have significant differences in the population between the high and low areas of gastric carcinoma,and no association was found between 0901,1401,*1701 and gastric cancer susceptibility and infection susceptibility of Hp.更多还原