【摘要】 目的:研究小干扰RNA对人胃癌SGC7901细胞Survivin表达及细胞生长的抑制作用。方法:将小干扰RNA转染给胃癌SGC7901细胞来敲除Survivin的表达。用逆转录聚合酶链式反应(RT-PCR)和Western blotting测定Survivin mRNA和蛋白的表达。用甲基噻唑基四唑(MTT)来分析Survivin小干扰RNA对癌细胞的生长抑制作用。用流式细胞术检测SurvivinsiR-NA对细胞周期和细胞凋亡的影响。结果:小干扰RNA能有效而稳定地抑制胃癌SGC7901中Survivin表达,下调Survivin可以在体外显著抑制肿瘤细胞的生长,在转染后24、48和72h,转染Survivin特异性siRNA组生长抑制率显著高于对照组,用特异性SurvivinsiRNA处理后,凋亡率为13.56%,并出现G0/G1期阻滞。结论:下调Survivin可以在体外显著抑制肿瘤细胞的生长,抑制Survivin的表达可以诱导胃癌SGC7901细胞凋亡,Survivin特异性siRNA的运用作为一种治疗癌症的新途径值得进一步研究。更多还原

【Abstract】 OBJECTIVE:To investigate the inhibitory effect of small interfering RNA on the expression of Survivin and cell growth in human gastric carcinoma cell line SGC-7901. METHODS: Small interfering RNA was transfected into SGC-7901cells to knockdown the Survivin expression. mRNA and protein levels of Survivin were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The cell growth inhibition was determined by a methyl thiazolyl tetrazolium (MTT) assay. The effect of Survivin siRNA on cell cycle distribution and cell apoptosis were assayed by flow cytometry (FCM). RESULTS: RNA interference efficiently and stably suppressed the Survivin expression in SGC-7901 cells, and downregulation of Survivin resulted in significantly inhibition of tumor cell growth in vitro. At 24 h, 48 h, 72 h after the transfection, the growth inhibitory rates were significantly higher in specific Survivin siRNA group than those in the control. The apoptosis rate was 13.56% after the treatment of specific Survivin siRNA, and G0/G1 phase arrest was shown. CONCLUSIONS: Downregulation of Survivin results in significant inhibition of tumor growth in vitro. Inhibition of the expression of Survivin can induce apoptosis of SGC-7901 cells, and the use of Survivin specific siRNA deserves further investigation as a novel approach to cancer therapy.更多还原