【摘要】 目的研究氟尿嘧啶(5-Fluorouracial,5-FU)体外影响人大肠癌Lovo细胞增殖抑制及诱导凋亡的分子机制。方法采用四甲基偶氮唑盐(MTT)显色法,检测不同浓度5-FU作用不同时间对Lovo细胞生长所产生的不同影响;光镜观察凋亡细胞的形态特点;流式细胞术(FCM)检测凋亡细胞百分率;琼脂凝胶电泳、免疫组化SP法检测Lovo细胞增殖核抗原Ki-67及凋亡相关基因蛋白p53、Fas表达水平。结果5-FU能抑制Lovo细胞生长,在一定剂量和时间范围内,通过光镜观察,见凋亡细胞明显增多,经5-FU诱导后,用FCM分析Lovo细胞,发现凋亡百分率增加并显示剂量和时间效应。实验组细胞Ki-67表达明显低于对照组,Fas表达明显升高,而P53在诱导前后均无表达。结论5-FU能诱导Lovo细胞凋亡并抑制其增殖及侵袭性,Fas基因激活可能是触发Lovo细胞凋亡的主要机制。更多还原

【Abstract】 Objective To study the molecular mechanism for 5-Fluorouracial(5-FU) and clarify whether it inhibit proliferation and induce apoptosis of colon cancer Lovo cells.Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells;photomechanical method used to observe configuration and characteristics of apoptosis cells;FCM employed to test the percentage of apoptosis cells;electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.Results 5-FU inhibits growth of Lovo cells;it is found that within a limited dosage and period of time,there has been an obvious increase in apoptosis cells under the photomechanical scope;the analysis of Lovo cells by FCM showed that after the induction of 5-FU,the percentage of apoptosis cells has increased;and FCM also displays dosage and time effects.Electrophoresis was obviously in a stripe shape of trapeziform.In the project group,Ki-67 expression in cells is clearly lower than that in the control group,and Fas expression in cells apparently tends to rise,whereas P53 has no expression before and after the induction.Conclusion 5-FU can induce apoptosis of Lovo cells and inhibit its proliferation and attack;activation of Fas gene might be the primary mechanism of triggering Lovo cells to apoptosis.更多还原