【摘要】 为研究给水管网管壁微生物膜的群落结构,采用异养菌平板计数(HPC)和单链构象多态性(SSCP)技术,分析了北京市第九水厂管龄接近,管材和余氯浓度不同的2个样品异养菌数目和种类。细菌计数结果表明,2个样品微生物数量有较大差异。SSCP电泳及测序结果则显示2个样品中4条相同条带与蜡状芽孢杆菌、假单胞菌、苏云金芽孢杆菌和溶血不动杆菌的同源性分别为100%、99%、100%和97%。另一条只在第2个样品中出现的条带测序与未培养细菌(GenBank登录号EF09462)的同源性为96%。潜在致病菌的存在警示应更加重视饮用水的微生物安全性。更多还原

【Abstract】 Single strand conformation polymorphism(SSCP) and heterotrophic bacterial plate counts were used to identify the compositions of two biofilm samples,S1 and S2,from the Beijing 9th Waterworks Pipe Network.The pipes have similar ages but diffrent pipe materials and different residual chlorine concentrations.The results show that the two samples had different heterotrophic bacterial counts.The SSCP electrophoresis bands were sequenced and contrasting with Genbank Data show that the two samples had 100% homology with Bacillus cereus,99% with Peudomonas sp.yged143,100% with Bacillus thuringiensis,and 97% with Acinetobacter haemolyticus strain AR-46.The band observed only in the S2 sample had 96% homology with the uncultured bacterium clone.These potential pathogens require that more attention be paid to the microbiology safety of the Beijing drinking water.更多还原