【摘要】 将提纯的淋巴囊肿病毒(LCDV)接种于牙鲆鳃细胞系(FG),可以观察到明显的细胞病变,半数细胞培养物感染量(TCID50)为22.88/40μL。微量细胞病变中和实验显示患淋巴囊肿病的牙鲆血清以及通过免疫获得的兔抗LCDV血清对LCDV感染都具有明显的中和作用,具有中和效果的血清最高稀释度分别为1∶64和1∶160。本文建立的FG细胞检测LCDV中和抗体方法可以作为筛选LCDV中和抗体的有效途径,且重复性和稳定性较好。更多还原

【Abstract】 Obvious cytopathic effect could be found on flounder gill(FG)cells post inoculation with lymphocystis disease virus(LCDV),purified from lymphocystis cells of Japanese flounder Paralichthys olivaceus,and the half tissue culture infection dosage(TCID50)was 22.88 per 40 μL.Micro-cytopathic effect neutralization test(MCPENT)demonstrated that the infectivity to FG cells was reduced by pretreatment of virus with serum obtained from LCD-diseased Japanese flounder and rabbit anti-LCDV serum,the neutralization titers were respectively 1∶64 and 1∶160.As a sensitive cell line for LCDV,FG cells could be used in the detection of neutralizing antibodies to LCDV.更多还原