【摘要】 目的:探讨大鼠甲状旁腺细胞的培养方法及其分泌功能,为甲状旁腺细胞移植奠定实验基础。方法:胶原酶和胰蛋白酶系列消化甲状旁腺后,进行细胞培养。透射电镜检查培养5d的甲状旁腺细胞,对1、3、5、7d的细胞培养液行甲状旁腺激素测定。结果:培养5d的甲状旁腺细胞具有丰富的粗面内质网、高尔基复合体、杆状的线粒体和分泌颗粒。甲状旁腺细胞经过培养后可以正常分泌甲状旁腺激素,以第5天为最佳。结论:培养5d的甲状旁腺细胞仍保持原有的形态结构,分泌功能最强,可作为同种移植的最佳供体。更多还原

【Abstract】 Objective:To investigate the culture technique and secretory function of parathyroid cells in rats in order to establish experimental basis of parathyroid cell transplantation.Methods:After parathyroid glands were digested by collagenase and trypsin,isolated parathyroid cells were cultured.The cells cultured for 5 days were observed by transmission electron microscopy.Parathyroid hormone obtained from the cell culture fluid on day 1,3,5 and 7 was measured respectively.Results:Parathyroid cells cultured for 5 days had abundant rough surfaced endoplasmic reticula、Golgi bodies、stab mitochondria and secretory granules.Cultured parathyroid cells could secrete parathyroid hormone normally,especially on the 5th day.Conclusion:Parathyroid cells cultured for 5 days still possess original morphologic structure and have maximum secreting function,so it is an optimal donor for transplantation.更多还原