【摘要】 双效菌素(zwittermicin A,ZwA)是一种氨基多元醇类新型广谱抗生素,zmaR为其抗性基因。在苏云金芽胞杆菌(Bacillus thuringiensis)YBT-1520菌株ZwA合成基因簇末端存在一个类似ABC transporter的序列(命名为zwa-FEG)。将zwa-FEG基因在大肠杆菌(Escherichia coli)中表达,大肠杆菌能获得ZwA的抗性;将zwa-FEG转移到产ZwA的蜡状芽胞杆菌(Bacillus cereus)菌株UW85中,能使其ZwA产量显著提高。推测zwa-FEG是一种ZwA专一性的ABC transporter,它能将ZwA分泌到胞外,从而增强ZwA的合成和ZwA的抗性。更多还原

【Abstract】 Zwittermicin A (ZwA) is a novel antibiotic which shows diverse inhibitory activity against a broad target of plant pathogens, and zmaR is its resistant gene. Analysis of synthesis gene cluster of ZwA in Bacillus thuringiensis YBT-1520 showed that there was an operon (zwa-FEG)with highly similarity to ABC transporter at the downsteam of the gene cluster. When the zwa-FEG was transferred to Escherichia E. coli DH10B, the recombinant obtained the resistantce of ZwA. After the zwa-FEG was transferred to ZwA-producing strain Bacillus cereus UW85, the production of ZwA was improved. It suggested that zwa-FEG was a cluster cod- ing ABC transporter which is ZwA specific, and can improve the resistance of ZwA by transporting ZwA to outside of the cell.更多还原