【摘要】 利用筛选出的18对SSR引物分析了来自我国的栽培小豆(Vigna angularis(Willd.)Ohwi & Ohashi)优异种质53份和日本引进种质27份。结果表明,在所有参试的80份小豆种质中共鉴定出等位变异92个,平均每个位点为5.1个;其中53份国内小豆和27份日本小豆的等位变异数分别为89个和74个,平均每个位点分别为4.9个和4.1个。平均每个位点的多态信息含量(polymorphic information content,PIC)为0.64,变化范围为0.23～0.83。其中国内小豆的平均PIC为0.63,变化范围为0.23～0.86;日本小豆平均PIC为0.61,变化范围为0.20～0.81。国内栽培小豆和日本小豆在等位变异数、PIC和遗传相似性系数均存在差异,UPGMA聚类分析将80份小豆明显分为5大类,聚类结果与小豆种质地理起源呈现出较高的相关性。更多还原

【Abstract】 The eighteen pairs of SSR primers were screened and used to analyze the genetic diversity of 80 accessions of azuki bean (Vigna angularis), 53 collected from China and 27 from Japan. A total of 92 alleles were detected, among them 89 and 74 were detected in Chinese and Japanese azuki bean, respectively. The average number of alleles per locus was 5.4 for all the accessions, 4.9 for Chinese azuki bean and 4.1 for Japanese azuki bean. The polymorphic information content (PIC) of each SSR locus varied from 0.23 to 0.83 with an average of 0.64 in all the accessions, from 0.23 to 0.86 with an average of 0.63 in the Chinese azuki bean, and from 0.20 to 0.81 with an average of 0.61 in the Japanese azuki bean. Differences were detected for specific alleles, PIC and pairwise genetic similarity between Chinese and Japanese accessions of azuki bean. Based on the similarity data the 80 accessions were clustered into five groups, and the dendrogram generally agreed with their geographic origin.更多还原