【摘要】 选用两种对稀有密码子效应不同的表达宿主菌大肠杆菌(Escherichia coli)BL21(DE3)和Rossetta(DE3),和两种不同的表达载体pET28a(+)和pGEX-KG,构建出4种重组表达菌(pET-fas GBL21(DE3)、pET-fasG Rossetta(DE3)、pKG-fasGBL21 (DE3)和pKG-fasG Rossetta(DE3))来考查稀有密码子对fasG基因表达的影响。对重组表达菌进行诱导表达的结果表明,不管是pET-fasG质粒还是pKG-fasG质粒,目的蛋白在Rossetta(DE3)中的表达量都要比在BL21(DE3)中的高。使用补充了额外的稀有密码子tRNA的表达菌Rossetta(DE3),提高了目的蛋白的表达量。更多还原

【Abstract】 Four bioengineered bacteria of fasG gene were constructed using Two Escherichia coli expression sWains(BL21(DE3) and Rossetta(DE3))with different tRNA profiles and two E.coli expression vectors(pET28a(+)and pGEX-KG)to examine the influence of the rare codons on the expression of fasG gene.The result of inducing expression of the bioengineered bacteria showed that the expression levels of target proteins in Rossetta(DE3)were higher than that in BL21(DE3),either using pET-fasG or pKG-fasG. And the expression levels of target proteins were enhanced using the expression sWain Rossetta(DE3)which contained the tRNA of rare codons.更多还原