【摘要】 以盆栽3年生红富士苹果(Malus domestica Borkh cv.Rehd.Fuji)做试材,研究了土壤控水和自然失水过程中,甜菜碱(Bet)、β-氨基丁酸(β-ABA)和壳聚糖(OC)以及自然干旱(ND)对叶片水分利用效率(WUE)和脯氨酸含量的影响。结果表明,在土壤相对含水率(RWC)70%～80%时,β-ABA和OC处理后第2天叶片脯氨酸含量升高、第10天叶片WUE升高。在自然失水过程中,预先Bet、β-ABA和OC的叶片脯氨酸含量明显升高。自然失水第5天(RWC=50%～60%),经自然干旱(ND)预处理的植株,叶片WUE最高,其次是经Bet预处理的,再次是OC;自然失水第10天(RWC=25%～35%),叶片WUE普遍下降,但经Bet、β-ABA和OC预处理的明显高于对照。综合比较显示,Bet、β-ABA、OC和ND预处理都能不同程度地诱导苹果叶片积累脯氨酸,并可有效延缓严重干旱胁迫引起的叶片WUE下降,其中甜菜碱效果最好,其次是β-氨基丁酸。更多还原

【Abstract】 Three-years-old apple trees(Malus domestica Borkh c.v.Rehd Fuji) were used as materials in this experiment.It was studied that the effect of Betaine(Bet),β-aminobutyric acid(β-ABA),Oligo-Chitosan(OC) and natural drought(ND) on leaf proline accumulation and water use efficiency(WUE) under controlling water treatments and natural water-losing of soil.The results show that β-ABA and OC increase proline content in secourd day with treatment and increas WUE on tenth day under RWC 70%～80%.The treatments of Bet,β-ABA,OC and ND all increase the content of proline in leaves during water-losing of soil.On fifth day of water-losing(RWC=50%～60%),leaf WUE is the highest in the plant treated by ND,in that by Bet secondly and OC thirdly.On tenth day of water-losing(RWC=50%～60%),leaf WUE decreas generally;the WUE of leaf pre-treated by Bet,β-ABA and OC all are higher than that by control significantly.Those results suggest that the pretreatments of Bet,β-ABA,OC and ND all can induce the accumulation of proline in a certain degree and resist the decrease of leaf WUE under severe water stress,in which effect of Bet is best and β-ABA secondly.Betaine can increase effectively the drought resistance and leaf WUE of apple tree.更多还原