【摘要】 为研究半胱胺对仔猪血清促生长类激素水平及不同组织中生长激素受体(GHR)表达的影响,选取临床检查健康的90头新生"杜×长×大"三元杂交仔猪,平均体重为(4.56±0.18)kg,随机分成3组,每组30头。在14日龄,分别给对照组、试验Ⅰ组、Ⅱ组仔猪饲喂基础日粮、基础日粮+120 mg/kg半胱胺及基础日粮+200 mg/kg半胱胺。试验期31 d。在14、24、32和45日龄,从每组分别选取10头仔猪经前腔静脉采血,测定血清GH、SS、T3、T4和IGF-Ⅰ水平。在45日龄时,每组选6头仔猪,屠宰取样,采用反转录多聚酶链式反应(RT-PCR)方法,定量分析组织中GHR mRNA的相对丰度。结果表明,半胱胺可以显著提高血清中GH、T3、T4、IGF-Ⅰ水平,降低血清SS含量,增加肝脏、胸腺及脾脏组织GHR mRNA表达量。日粮中添加适量半胱胺能有效地提高仔猪血清促生长类激素水平及组织中生长激素受体的表达,缓解断奶应激。更多还原

【Abstract】 The objectivity of the experiment was to study the effects of cysteamine on growth-promoting hormone levels in serum and expression of the GH receptor mRNA in tissues of piglets,and further explore the mechanism of cysteamine stimulating growth.Ninety newborn "Duroc×Landrace×Yorkshire" crossbred healthy piglets were randomly divided into 3 groups of 30 piglets each;the average weight was(4.56±0.18)kg.At 14 days of age,the piglets in Control group,Group Ⅰ and Group Ⅱ were fed with basal diet,basal diet+120 mg/kg CS or basal diet +200 mg/kg CS,respectively.Serum samples of 10 piglets from each group were taken from the anterior vena cava to measure the levels of GH,SS,T3,T4 and IGF-Ⅰ on d 14,24,32 and 45,respectively.The trial was conducted for 31 d.Six piglets from each group were slaughtered at 45 days of age,and the tissues were sampled to measure the contents of GHR mRNA by RT-PCR.The results indicated that cysteamine decreased significantly the levels of serum SS,increased the levels of serum GH,T3,T4 and IGF-Ⅰ as well as the expression of GHR mRNA in the liver、thymus and spleen of piglets.This study showed that the diet containing CS can improve growth-promoting hormone levels in serum,as well as the expression of the GH receptor mRNA in tissues in piglets;it also efficiently relieves the weanling stress on piglets.更多还原