【摘要】 目的探讨胡芦巴的降脂机制,观察经胡芦巴水溶液处理后,Caco-2细胞中NPC1L1(胆固醇吸收的主要载体基因)的mRNA表达水平。方法将胡芦巴水溶液暴露于Caco-2细胞中24、42、48小时,未处理组作为对照组,从细胞中分离mRNA进行Real-Time PCR分析,采用ABI PRISM7000生物程序分析系统测定Caco-2细胞的NPC1L1的mRNA表达水平。结果经胡芦巴水溶液处理的Caco-2细胞NPC1L1的mRNA表达水平与对照组(未治疗组)相比降低47%。结论胡芦巴可降低Caco-2细胞的NPC1L1的mRNA表达水平。更多还原

【Abstract】 Objective To investigate the blood lipid lowering effect mechanism of Fenugreek(huluba) by observing the expression of NPC1L1 mRNA of cultured Caco-2 cells in huluba water-soluble extracts.MethodsThe Caco-2 cell was exposed to water-soluble extracts of the huluba seeds for 24,42 and 48hrs,the cell group which is no exposure to huluba as the control group,then isolated mRNA from cells to carry on the Real-Time PCR analysis,investigate the expression level of NPC1L1 mRNA of cultured Caco-2 cells with ABI PRISM 7000 Sequence Detection System.ResultsThe NPC1L1 mRNA expression was lower(47%) than that in control(no exposure) group.ConclusionThe huluba has lipid lowering effect through the expression of NPC1L1 mRNA in Caco-2 cell.更多还原