【摘要】 目的观察氧化苦参碱对ConA诱导的小鼠急性免疫性肝损伤的干预效果。方法将Balb/c小鼠100只随机分为4组:正常对照组(A组)尾静脉注射生理盐水0.3ml,氧化苦参碱对照组(B组)腹腔注射氧化苦参碱注射液60mg/kg,1次/天,连续2天。肝损伤组(C组)尾静脉注射ConA 12.5mg/kg,氧化苦参碱干预组(D组)在制作肝损伤模型的同时,给予氧化苦参碱注射液,方法和计量同B组。于造模8小时、24小时和72小时观察肝的组织学;IL-1、TGF-β1免疫组化变化;检测血清ALT、AST值,并对结果进行分析。结果①C组肝脏组织结构破坏严重,D组各时间段的肝组织结构损伤程度均轻于C组;②IL-1、TGF-β1免疫阳性细胞表达D组高于C组。③血清ALT、AST值C组和D组均高于A组和B组,P(0.01,尤其是8小时时,D组高于C组。但24小时和72小时各项指标趋于下降。结论氧化苦参碱具有抗急性免疫性肝损伤的功能,但早期使用可能会产生一过性肝功能损伤。更多还原

【Abstract】 Objective To investigate intervention effects of Oxymatrine in ConA-induced autoimmune acute liver injury in mice in the process.Methods Balb/c mice were randomly divided into control group,the Oxymatrine control group,liver injury group and Oxymatrine treatment group.Only saline 0.3 ml was injected via tail vein in normal control group,ConA12.5mg/Kg was injected via tail vein in liver injury group,Oxymatrine 60 mg/Kg was injected via intraperitoneal in Oxymatrine group,Oxymatrine was injected via tail vein half an hour before intraperitoneal injection 60 mg/Kg of ConA in the treatment group,every 24 hours once.Liver morphology and immunohistochemical(IL-1,TGF-β1)positive cells in the liver expression changes of serum ALT and AST values of 8,24,72 hours were observed.Results Liver tissue structure was significantly damaged after ConA injection.Morphology damaged in Oxymatrine treatment group at three different stages was significantly lighter than that in liver injury group.Serum ALT and AST in liver injury group and the Oxymatrine treatment group was significantly higher than the normal control group and the control group Oxymatrine(P﹤0.01),especially when Oxymatrine was injected after 8 hours in treatment group was higher than that of liver injury group.Immunohistochemical IL-1,TGF-β1 positive cells was higher than that of liver injury group,but after 24 h and 72 tend to decline.Conclusion Oxymatrine has anti-immune liver injury effects in acute liver injury,but in experimental autoimmune acute liver injury may have transient liver injury in early period of acute liver injury.更多还原