【摘要】 目的对在BALB/c小鼠附睾精子中内质网蛋白29(endoplasmic reticulum protein 29,ERp29)进行鉴定以及定位。方法应用免疫印迹方法鉴定BALB/c小鼠附睾头、尾部精子ERp29蛋白,同时利用激光共聚焦显微镜和间接免疫荧光定位的方法研究ERp29在附睾头、尾部精子上的定位。结果证实ERp29蛋白存在于BALB/c小鼠附睾头部和尾部精子中,且其在尾部精子中含量较头部明显增高。ERp29蛋白主要定位于BALB/c小鼠附睾头部精子的头部前端,而在附睾尾部精子则主要定位于头部和尾部主段。结论通过对ERp29蛋白在BALB/c小鼠附睾头部和尾部精子上的鉴定和定位,可以帮助进一步研究该蛋白对小鼠精子的作用。更多还原

【Abstract】 Objective To identify and localize ERp29 in spermatozoa from BALB/c mouse epididymal caput and cauda.Methods ERp29 was identified by Western Blot,and localized by indirect immunofluorescence employed laser scanning confocal microscopy.Results ERp29 was present in BALB/c mouse spermatozoa from epididymal caput and cauda,which was mostly localized on the partial region of sperm head from epididymal caput;however,it was localized on the whole sperm head and tail region (principal piece) from cauda.Additionally,the spermatozoa from epidiymal cauda contained more ERp29 protein compared with that in spermatozoa from the caput.Conclusion ERp29 might play an important role in mouse sperm maturation during epididymal transit.更多还原