【摘要】 试验利用SDS-聚丙烯酰胺凝胶电泳技术对牛乳进行快速质量检测研究,通过对样品稀释倍数、电压条件、染色时间、漂洗时间及次数等条件进行摸索,获得了条带清晰的牛乳蛋白电泳图谱。结果表明,快速聚丙烯酰胺凝胶电泳法鉴定牛乳蛋白质的最佳条件是,用8%分离胶在120V电压下电泳,使用超级灵敏度蛋白质电泳快速染液染色,煮沸染色1min,振荡10min,可实现快速鉴定牛乳蛋白的目的。更多还原

【Abstract】 The SDS-polyacrylamide gel electrophoresis was taken to the rapid detection research on milk quality. Through the exploration on conditions such as dilution multiple, voltage conditions, dyeing time, washing time and times, and the milk protein electrophoretogram with clear bands was obtained. The test result showed that, the optimum condition of milk protein identification with rapid polyacrylamide gel electrophoresis was that 8 % separation gel was used for electrophoresis under 120 V and the protein electrophoresis dyeing solution with super sensitivity was taken to rapidly stain, with boiling for 1 min and shaking for 10 min, which could realize the aim of rapid identification of milk protein.更多还原