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超声波提取紫花地丁总黄酮及含量测定
Extraction and Determination of Total Flavone from Viola Philippica Cav by Spectrophotometry
【摘要】 本文采用超声波辅助提取法提取紫花地丁中总黄酮,以芦丁为对照品,利用亚硝酸钠-硝酸铝-氢氧化钠体系分光光度法,在波长为(508 nm)处,测定样品中总黄酮的含量.紫花地丁中总黄酮的平均含量为26.55 mg/g,回收率范围为108%~109%.且超声波辅助提取法可以加快提取速度,提高提取效率.
【Abstract】 Using a method of ultrasonic wave extraction for extraction of total flavones in Viola philippica Cav.Using Dutin as a standard goods,the content of total flavonoids were determined by spectrophotometry,the detection wavelength was at 508nm and the system of NaNO2,Al(NO2)3,NaOH and(NH4)2Fe(SO4)2,H2O2,Phen.The total flavonoids content is 26.55mg/g and the recovery rate is 108%~109%.Ultrasonic wave can speed up the velocity of reaction and extract efficiency.
【关键词】 超声波法;
紫花地丁;
总黄酮;
分光光度法;
【Key words】 Ultrasonic wave extraction; Viola philippica Cav; Total flavone; Spectrophotometry;
【Key words】 Ultrasonic wave extraction; Viola philippica Cav; Total flavone; Spectrophotometry;
【基金】 内蒙古自治区自然科学基金项目(200607010209)
- 【文献出处】 内蒙古民族大学学报(自然科学版) ,Journal of Inner Mongolia University for Nationalities(Natural Sciences) , 编辑部邮箱 ,2008年05期
- 【分类号】TQ461
- 【被引频次】18
- 【下载频次】477