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几种影响沙芥愈伤组织培养的因素
FACTORS INFLUENCING ON CALLUS TISSUE CULTURE OF Pugionium cornutum
【摘要】 选用沙芥子叶和下胚轴为外植体,探讨了植物激素、苗龄、外植体切取部位及放置方式对沙芥愈伤组织的影响。研究结果表明:苗龄为12d~16d的外植体最佳,形态学上的中、上端子叶和下胚轴平放在B5+6BA1.0 mg.L-1+NAA0.5 mg.L-1培养基上诱导愈伤组织较好。
【Abstract】 We used the cotyledons and hypocotyls of Pugionium comutum for the explants,and discussed the influence of hormone combinations,ages of seedlings etc on callus of Pugionium comutum.The results showed that 12~16d seedlings were suited to induce callus,the optimum explants was middle and tip of cotyledons and hypocotyls,and they could be induced callus lying on medium B5+6BA1.0 mg·L-1 +NAA0.5 mg·L-1.
【关键词】 沙芥;
子叶;
下胚轴;
愈伤组织;
培养基;
【Key words】 Pugionium comutum; cotyledon; hypocotyl; callus; culture medium;
【Key words】 Pugionium comutum; cotyledon; hypocotyl; callus; culture medium;
【基金】 国家自然科学基金(30260067、30460080);内蒙古自然科学基金(200110802501、20030820513);内蒙古科技攻关项目(20060202、20050305)
- 【文献出处】 内蒙古农业大学学报(自然科学版) ,Journal of Inner Mongolia Agricultural University(Natural Science Edition) , 编辑部邮箱 ,2008年03期
- 【分类号】S647.602.4
- 【被引频次】4
- 【下载频次】105