【摘要】 目的:研究17β-雌二醇(E2)对小鼠生精细胞T型钙电流(ICaT)的影响。方法:采用膜片钳全细胞记录技术观察E2对急性机械分离的小鼠生精细胞ICaT的影响。结果:E2(1、10、100μmol/L)呈浓度、电压依赖性抑制小鼠生精细胞钙电流,抑制率分别为(13.48±1.86)%、(28.98±2.70)%和(52.93±3.42)%(n=6,P<0.05)。E2对T型钙通道的半数最大抑制浓度(K50)为8.89μmol/L。100μmol/LE2显著改变T型钙通道的激活和失活特性:半数激活电压(Va1/2)和激活斜率因子(κa)分别从(-48.94±0.22)mV、(5.19±0.19)mV变为(-54.34±1.02)mV和(6.02±0.84)mV(n=5,P<0.05);而半数失活电压(Vi1/2)和失活斜率因子(κi)分别从(-56.51±0.13)mV、(3.36±0.11)mV变为(-61.78±0.50)mV、(4.25±0.37)mV(n=5,P<0.05)。结论:E2对生精细胞T型钙通道有抑制作用,呈浓度依赖性。更多还原

【Abstract】 Objective:To investigate the effect of 17β-estradiol(E2) on T-type calcium currents(ICaT) in spermatogenic cells.Methods:Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells by the whole-cell patch clamp technique and the effects of E2 on ICaT were observed.Results:E2 at the concentrations of 1,10 and 100 μmol/L significantly inhibited ICaT in the mouse spermatogenic cells,with the KC50 value of 8.89 μmol/L and the inhibition rates of(13.48±1.86) %,(28.98±2.70) and(52.93±3.42)%,respectively(n=6,P < 0.05).E2 of 100 μmol/L significantly changed the activation and inactivation of ICaT:the half activation potential(Va1/2) and the activation steepness factor(κa) from(-48.94±0.22) mV and(5.19±0.19) mV to(-54.34±1.02) mV and(6.02±0.84) mV(n=5,P<0.05),and the half inactivation potential(Vi1/2) and the inactivation steepness factor(κi) from(-56.51±0.13) mV and(3.36±0.11) mV to(-61.78±0.50) mV and(4.25±0.37) mV,respectively(n=5,P<0.05).Conclusion:E2 has a significant inhibitory effect on ICaT in mouse spermatogenic cells in a concentration-dependent manner.更多还原