【摘要】 目的:利用三色荧光原位杂交方法分析弱精子症患者精子X,Y,18号染色体数目畸变。方法:应用X、Y、18号染色体探针对10例弱精子症患者和5例健康男性进行三色荧光原位杂交实验,检测其精子非整倍体数目畸变情况。结果:计数45547个精子,杂交率99.18%。双体类型为XX18、XY18、YY18、X1818、Y1818。病例组各类双体率分别为(0.124±0.086)%、(0.360±0.380)%、(0.109±0.195)%、(0.342±0.746)%、(0.299±0.564)%。对照组各类双体率为(0.014±0.019)%、(0.090±0.080)%、(0.030±0.031)%、(0.068±0.103)%、(0.075±0.083)%。病例组的非整倍体率9.25%,对照组为2.70%,两者比较,差异有显著性(P(0.01)。结论:弱精子症患者的精子比对照组精子的染色体非整倍体率高,为提供更有效的科学依据,尚需大样本研究。更多还原

【Abstract】 Objective: To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization. Methods: The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa. Results: Of the 45 547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124±0.086)%, (0.360±0.380)%, (0.109±0.195)%, (0.342±0.746)% and (0.299±0.564)% in the case group and (0.014±0.019)%, (0.090±0.080)%, (0.030±0.031)%, (0.068±0.103)% and (0.075±0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P<0.01). Conclusion: Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.更多还原