【摘要】 应用 HepG2细胞作为研究对象,探讨 Fe₂O₃纳米粒子对肿瘤细胞的活力与凋亡的影响.采用四甲基偶氮唑盐（MTT）还原检测法确定受试物的染毒剂量.分别对各个组进行流式细胞术检测细胞线粒体膜电位变化及凋亡.结果显示,Fe₂O₃纳米粒子在0.555～3.310 mg/mL 范围内均可抑制 HepG2细胞增值,IC₅₀为1.1lmg/mL.Fe₂O₃纳米粒子在0.173、0.347、0.694 mg/mL 剂量组均导致细胞线粒体膜电位均较对照组有所下降且细胞凋亡率显著增高.更多还原

【Abstract】 The adverse effects of proliferation and apoptosis and the oxidative damage on HepG2 cells induced by Fe₂O₃ nanoparticles were studied.The cytotoxicity and the intervening concentration of nanoparticles were detected by MTT reduction assay.The mitochondrial membrane potent（MMP）and cell apoptosis rate of different exposure group were analyzed by fluorospectrophotometry,respectively.The results indicated that the proliferations of HepG2 cells were suppressed when exposed to Fe₂O₃ nanoparticles of 0.555～3.310 mg/mL.The IC₅₀ of Fe₂O₃ nanoparticles on HepG2 cells was 1.11 mg/mL.The flow cytometry assay showed that the MMP decreased in different exposure groups and cell apoptosis rate of exposure groups were higher than that of the control group.It can be concluded that Fe₂O₃ nanoparticles could reduce the MMP and lead to apoptosis in HepG2 cells.更多还原