【摘要】 目的:探讨As2O3与阿司匹林联合应用对人胃癌SGC-7901细胞生长的影响。方法:采用MTT法检测As2O3和阿司匹林对SGC-7901细胞株增殖的影响,采用流式细胞术检测两者对SGC-7901细胞凋亡和细胞周期分布的影响。结果:①药物作用后72h,4μmol/L和2mmol/LAs2O3组生长抑制率分别为31.04%及16.11%,与阴性对照组相比差异具有统计学意义(P<0.05);2μmol/LAs2O3+1mmol/L阿司匹林联合用药组的生长抑制率为24.19%,与阴性对照组、2μmol/LAs2O3(5.96%)及1mmol/L阿司匹林(4.86%)相比,差异均有统计学意义(P<0.05);②2μmol/LAs2O3与1mmol/L阿司匹林联合组细胞的凋亡率是19.01%,与阴性对照组(1.55%)、2μmol/LAs2O3组(1.81%)及1mmol/L阿司匹林组(1.64%)相比,差异均有统计学意义(P<0.05);③As2O3使细胞G0/G1期比例下降,G2/M期比例增加。阿司匹林使细胞G0/G1期比例增加,S和G2/M期比例下降。2μmol/LAs2O3与1mmol/L阿司匹林联合应用后72h,G0/G1期和G2/M期比例增加,S期比例下降。结论:As2O3、阿司匹林可以抑制SGC-7901细胞增殖、诱导其凋亡,阻滞细胞周期,两者联合应用具有协同作用,可以同时降低两药的用量。更多还原

【Abstract】 Objective:To investigate the inhibitory effects of As2O3 and Aspirin on cell proliferation in human gastric carcinoma cells(SGC-7901 cell lines).Methods:The inhibition of proliferation,apoptosis and cell cycle of gastric cancer cells SGC-7901 were detected by MTT assay and the flow cytometry and light microscope.Results:①After 72 hours,MTT assay showed the growth of the SGC-7901 cells was inhibited by As2O3(4 μmol/L),Aspirin(2 mmol/L),and As2O3+Aspirin(2 μmol/L + 1 mmol/L).And the inhibitory rates were 31.04%,16.11% and 24.19% respectively.It also showed the inhibitory effects among negative control group,two single drug groups(As2O3 group,Aspirin group) and drug combination group(P < 0.05).②Flow cytometry showed the apoptosis rates were 1.55% in negative group,1.81% in As2O3 group,1.64% in Aspirin group and 19.01% in As2O3+Aspirin group,which showed statistical significance(P < 0.05).③The proportion of cells at G0/ G1 phase was significantly decreased while the proportion of cells at G2/ M phase was increased in As2O3 group.The proportion of cells at G0/ G1 phase was significantly increased while the proportion of cells at S phase and G2/ M phase were decreased in Aspirin group.And the increasing of proportion of cells at G0/ G1 phase and G2/ M phase were found in drug combination group,while proportion of cells at S phase decreased.Conclusion:As2O3 and Aspirin could inhibit the growth of gastric cancer cell SGC-7901 by inducing apoptosis and cell cycle arrest.It showed that inhibitory effect on proliferation of SGC-7901 cell line in drug combination group(As2O3+Aspirin) were markedly enhanced and their dose was decreased.更多还原