【摘要】 目的:通过构建饮食诱导肥胖小鼠模型,观察吡格列酮对脂肪细胞因子脂联素表达的影响,探讨过氧化物酶体增殖物激活受体(PPAR)-γ激动剂在胰岛素抵抗及2型糖尿病中的作用机制。方法:对肥胖组小鼠予吡格列酮(每日10mg/kg)灌胃14天后处死,放免法测小鼠空腹胰岛素水平,生化法检测血糖,ELISA法检测血清脂联素含量,实时定量PCR检测脂肪组织脂联素的mRNA表达。结果:肥胖组小鼠体重,空腹胰岛素及血糖水平明显升高(P<0.05)。给药后,肥胖干预组小鼠空腹胰岛素降低(P<0.05),血糖降低(P<0.01),血清脂联素含量升高(P<0.01),HE染色显示脂肪细胞体积明显缩小,脂肪组织脂联素mRNA表达升高(P<0.05)。结论:PPAR-γ激动剂通过影响脂肪细胞因子的表达,从而改善胰岛素抵抗。更多还原

【Abstract】 Objective:To investigate the mechanism of Pioglitazone(PGZ) on insulin resistance and type 2 diabetes mellitus by using the DIO(diet-induced obese) mice model.Methods:The C57BL/6J mice were randomly divided into the normal diet group and high-fat diet(HFD) group.After 20 weeks,the obese mice were randomly divided into obese control group,PGZ group.They were orally administered placebo and PGZ[10 mg/(kg·d)] respectively for two weeks.The adiponectin mRNA expression levels from adipose tissue were analyzed by real-time quantitative PCR.The levels of plasma glucose,serum insulin and adiponectin were measured by Biochemistry technology,Radioimmunity and ELISA.The adipocyte sizes were also observed by Immunohistochemistry.Results:The weight,plasma glucose and serum insulin levels increased(P < 0.05) in HFD group,and the adipocyte sizes were bigger.Comparing to obese controls,serum insulin and glucose levels significantly decreased(P < 0.05) and the adipocyte sizes reduced in PGZ treatment group,while plasma adiponectin level raised(P < 0.01).Moreover,the mRNA expressions of adiponectin upregulated(P < 0.05).Conclusion:The effects of PPAR-γ agonist on insulin resistance can be carried out through regulating the expression of adipocytokines.更多还原