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山楂果实中黄酮类化合物的超高压提取研究
Extract Flavonoids of Hawthorn by Ultrahigh Pressure
【摘要】 用常温超高压技术提取山楂中黄酮类化合物,为了研究提取的最佳工艺,采用正交试验优化,利用分光光度法检测提取液中总黄酮含量。结果表明,超高压提取山楂果中黄酮类化合物的最佳提取工艺参数分别为:提取溶剂为50%乙醇,料液比为1:40,浸泡时间2h,提取压力300MPa,提取时间为3min。超高压提取时间短,提取液澄清稳定。
【Abstract】 The ultrahigh pressure extraction method was used to extract flavonoids from Hawthorn at normal temperature.The content of flavonoids was determined by UV-spectrophotometry.Parameters that may influence the extraction process were investigated.The orthogonal test was applied to obtain the optimum extraction condition,which was as follows:solvent of 50% ethanol,ratio of raw Hawthorn(g) to solvent(mL) of 1:40,marinate time of 2 hours,extraction pressure of 300mPa and extraction time of 3 min.Results of this work show that the ultrahigh pressure extraction method has several advantages,such as shorter extraction time,and less impurity in the extracted flavonoids.
【Key words】 hawthorn; flavonoids; ultrahigh pressure extraction; orthogonal design;
- 【文献出处】 农机化研究 ,Journal of Agricultural Mechanization Research , 编辑部邮箱 ,2008年07期
- 【分类号】TQ463.24
- 【被引频次】5
- 【下载频次】374