【摘要】 目的:探讨添加丙氨酰谷氨酰胺(Ala-Gln)全胃肠外营养(TPN)对烫伤大鼠空肠黏膜及蛋白质代谢的影响。方法:将33只SD大鼠随机分为非烫伤正常参照组(正常组)、传统TPN组(传统组)和添加二肽TPN组(二肽组)3组,每组11只。正常组自由饮食,另两组大鼠在颈外静脉插管后行30%体表面积Ⅲ度烫伤,并接受等热量(780kJ·kg-1·d-1)等氮量(氮量1.8g·kg-1·d-1,二肽组48%的氮量由Ala-Gln提供)TPN7d,留取24h尿测每日氮平衡和累积氮平衡;7d后取血测定总蛋白(TP)、白蛋白(ALB)、前白蛋白(PAB)、转铁蛋白(TRF),高效液相色谱法检测肌肉中谷氨酰胺(Gln)的含量;取一段空肠作黏膜形态学观察。结果:血清PAB、TRF及肌肉中Gln含量二肽组明显高于传统组(P<0.01);空肠黏膜绒毛高度、隐窝深度、黏膜厚度、绒毛表面积二肽组明显高于传统组(P<0.01)。结论:烫伤大鼠应用添加Ala-Gln的TPN能改善机体氮平衡,促进机体蛋白质合成,减缓肌肉中Gln含量的下降,减轻空肠黏膜的萎缩。更多还原

【Abstract】 Objective To evaluate the effect of alanyl-glutamine(Ala-Gln) supplemented of total parenteral nutrition(TPN) on intestinal mucosa and protein metabolism in burned rats.Methods Thirty-three SD rats were divided into three groups equally:chow group,conventional TPN group(convention group) and Ala-Gln supplemented TPN group(Ala-Gln group).The chow group received standard chow; the two TPN groups having been placed a central venous catheter subjected to 30% TBSA Ⅲ° burn and received isocaloric(780 kJ·kg-1·d-1 ) and isonitrogenous(N1.8 g ·kg-1·d-1,48% of nitrogen was supplied with Ala-Gln in Ala-Gln group) TPN for 7 days.Nitrogen balance(NB) and cumulated nitrogen balance(CNB) were monitored.At the end of TPN,serum total protein(TP),albumin(ALB),prealbumin(PAB),transferrin(TRF),Gln in muscle were monitored.Jejunal mucosal morphological changes were observed under light microscope.Results Serum PAB,TRF,Gln level in muscle and CNB in Ala-Gln group were significantly higher than those in convention group(P<0.01).The jejunal villus height,crypt depth,mucosal thickness and villous surface area increased significantly in Ala-Gln group.Conclusion Ala-Gln supplemented TPN in burned rats can promote protein anabolism,improve nitrogen balance,attenuate the decline in Gln in muscle and intestinal mucosal atrophy.更多还原