【摘要】 通过室内筛选获得了麦长管蚜对吡虫啉的抗性品系(25.22倍),并利用增效试验和解毒酶活力测定,对其抗性机理进行了研究。增效试验结果表明:顺丁烯二酸二乙酯在抗、感品系中对吡虫啉都没有明显的增效作用,但氧化胡椒基丁醚和磷酸三苯酯在2个品系均有增效作用,而且在抗性品系中的增效比(3.49和2.62)显著大于敏感品系(1.59和1.29)。解毒酶活力测定发现,抗、感品系的谷胱甘肽S-转移酶比活力没有显著差异,但抗性品系中羧酸酯酶的比活力是敏感品系的1.53倍(P<0.05)。综合分析认为,多功能氧化酶和羧酸酯酶活力增强在该麦长管蚜品系对吡虫啉的抗性中起重要作用。更多还原

【Abstract】 A resistant strain(about 25.22-fold)of Sitobion avenae to imidacloprid was developed in the laboratory.Synergism experiments and detoxification enzyme analysis were carried out to reveal the resistance mechanisms.The results showed that piperonyl butoxide and triphenyl phosphite had significant synergism on imidacloprid in both the resistant and susceptible strains,but diethyl maleate had not.The synergism ratio of piperonyl butoxide and triphenyl phosphite in the resistant strain were much higher(3.49 and 2.62,respectively)than in the susceptible strain(1.59 and 1.29,respectively).The resistant strain showed higher activity of carboxylesterases(about 1.53-fold)than the susceptible strain,but the activity of gluathione S-transferases was similar.These results indicated that the enhancement of the activity of carboxylesterase and mixed function oxidase should contribute to the imidacloprid-resistance in this Sitobion avenae strain.更多还原