【摘要】 目的探讨MSCs对GVHD的作用及其机制。方法建立大鼠同种异体骨髓移植模型,同时输入供者的T淋巴细胞诱导出移植物抗宿主反应,联合或不联合移植供体来源的MSCs,观察受鼠的生存时间,同时利用RT-PCR法研究Th1/Th2淋巴细胞亚群的比例,用ELISA法检测移植后体内IL-4细胞因子的浓度。结果GVHD组的平均生存时间为(17.30±2.33)天,实验组的平均生存时间为(24.10±2.36)天,与单独移植HSCs相比,MSCs与HSCs共移植明显延长的受鼠的生存时间。同时,GVHD组Th1/Th2细胞比值为1.29±0.06,IL-4因子的浓度平均为(14.84±2.59)pg/mL,实验组Th1/Th2细胞比值为(0.77±0.14),IL-4因子的浓度平均为(40.09±13.99)pg/mL。MSCs与HSCs共移植降低了体内Th1/Th2淋巴细胞亚群的比例,提高了体内IL-4细胞因子的浓度。结论MSCs与HSCs共移植能有效抑制HSCs移植后致死性GVHD的发生,延长生存时间,同时MSCs可能通过作用于体内Th1/Th2淋巴细胞亚群的比例,促进体内IL-4细胞因子的分泌从而间接发挥了抑制GVHD的作用。更多还原

【Abstract】 Objective To investigate the effects of bone marrow-derived mesenchymal stem cells(MSCs)on graft-versus-host disease(GVHD)after allogeneic hematopoietic stem cells transplantation(allo-HSCT)and the mechanisms.Methods The rat model of GVHD has been established by allogeneic HSCs with donor-derived T cells,with or without donor-derived MSCs co-transplantation.Effects of MSCs on GVHD were analyzed by median survival time of model rats.Ratio of Th1/Th2 T cells was assessed by RT-PCR and the concentration of IL-4 cytokine was assessed by ELISA.Results MSCs inhibited the lethal GVHD and increased the rats’ survival time after allo-HSCT.The median survival time was(17.30±2.33)d and 924.10±2.36)d in GVHD and experimental groups,respectively.Meantime,the ratio of Th1/Th2 T cells was 1.29±0.06 and 0.77±0.14 and the concentration of IL-4 cytokine was(14.84±2.59)pg/mL and(40.09±13.99)pg/mL in GVHD and experimental groups,respectively.The ratio of Th1/Th2 T cells was lower and the concentration of IL-4 cytokine was higher in experimental group than those in GVHD group.Conclusion MSCs may prevent the lethal GVHD after allo-HSCT by through Th1/Th2 T cells in vivo.更多还原