【摘要】 ①目的探讨大鼠肢体缺血再灌注(limbischemia-reperfusion,LIR)后牛磺酸对肝损伤及肿瘤坏死因子-α(TNF-α)的变化及其对肝损伤的保护效应。②方法实验采用Wistar大鼠40只,建立大鼠LIR损伤模型,随机分为对照(C)组,缺血再灌注(IR)组,牛磺酸(T)组和牛磺酸+缺血再灌注(TR)组,每组10只。分别测定各组大鼠血浆谷草转氨酶(ALT)、谷丙转氨酶(AST)和肝组织钙、MDA、XOD、髓过氧化物酶(MPO)及TNF-α含量,HE染色观察肝组织形态学变化。③结果与C组比较,IR和TR组血浆和肝组织ALT、AST、肝组织钙、MDA、XOD、MPO含量,TNF-α表达水平升高,但TR组水平较IR组显著降低。④结论牛磺酸可降低大鼠LIR引起肝损伤中TNF-α水平升高,减轻大鼠LIR所致肝脏损伤。更多还原

【Abstract】 Objective To observe the changes of liver injury and tumor necrosis factor alpha(TNF-α) after limbs ischemia-reperfusion in rats and the protective effect of taurine.Methods The model of limbs ischemia-reperfusion injury was established in 40 wistar rats and were randomly separated into 4 groups:control group(C),taurine group(T),ischemia-reperfusion group (IR)and taurine+ischemia-reperfusion group(TR),10 cases each group.The levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in plasma and the content of calcium,MDA,XOD,MPO and TNF-α in the plasma and the liver tissues were measured in each group,and the morphoiogic changes were observed with HE staining.Results Compared with the control group,the levels of ALT and AST in plasma and liver tissues,the contents of calcium,MDA,XOD,MPO and TNF-αincreased in liver tissues in IR group and TR group increased,while which in TR group were lower than those in IR group.Conclusion Taurine can decrease the level of TNF-αin the liver injury and mitigate the liver injury after limb ischemia-reperfusion injury in rats.更多还原