【摘要】 在吉林省长白山地区模拟检测了大气CO2浓度升高对土壤氮循环关键过程的影响。试验采用完全随机区组设计的开顶箱系统模拟环境CO2和高浓度CO2,起始于1999年春。选取长白山特有树种长白松(Pinus sylvestris var.sylvestri-formu),种子播种于1999年5月份,萌芽后开始CO2熏蒸处理。CO2熏蒸处理始于每年4月末止于10月末。分别在2006年6月、8月和2007年6月采集土壤样品,并检测土壤硝化酶(NEA)、反硝化酶(DEA)和固氮酶活性。结果表明,高浓度C02使土壤硝化酶(NEA)活性显著提高,提高幅度2006年6月为30.3%,2006年8月为30.9%,2007年6月为11.3%;土壤反硝化酶活性(DEA)在2006年6月份(P<0.012)和2006年8月份(P<0.005)被C02浓度升高显著抑制;在整个研究过程中没有发现C02浓度升高对固氮酶活性产生显著影响。因此,本研究认为C02浓度升高显著影响了土壤硝化酶(NEA)和反硝化酶活性(DEA)。图3表1参44。更多还原

【Abstract】 A study was conducted to determine the effects of elevated CO2 on soil N process at Changbai Mountain in Jilin Province, north-eastern China (42o24′N, 128o06′E, and 738 m elevation). A randomized complete block design of ambient and elevated CO2 was established in an open-top chamber facility in the spring of 1999. Changpai Scotch pine (Pinus sylvestris var. sylvestriformis seeds were sowed in May, 1999 and CO2 fumigation treatments began after seeds germination. In each year, the exposure started at the end of April and stopped at the end of October. Soil samples were collected in June and August 2006 and in June 2007, and soil nitrifying, denitrifying and N2-fixing enzyme activities were measured. Results show that soil nitrifying enzyme activities (NEA) in the 5–10 cm soil layer were significantly increased at elevated CO2 by 30.3% in June 2006, by 30.9% in August 2006 and by 11.3% in June 2007. Soil denitrifying enzyme activities (DEA) were significantly decreased by elevated CO2 treatment in June 2006 (P<0.012) and August 2006 (P<0.005) samplings in our study; no significant difference was detected in June 2007, and no significant changes in N2-fixing enzyme activity were found. This study suggests that elevated CO2 can alter soil nitrifying enzyme and denitrifying enzyme activities.更多还原