【摘要】 采用柱前NDA衍生的方法,建立了毛细管电泳电化学检测牛磺酸的分析方法。考察了NDA衍生条件、检测电势、分离电压、缓冲溶液pH值、缓冲溶液浓度等实验参数对牛磺酸检测的影响。在最佳实验条件下,检测牛磺酸的浓度范围是5.00×10-6~1.00×10-3mol/L,线性回归系数为0.9997,浓度检出限LOD c为1.60×10-6mol/L(S/N=3)。以此方法检测了人血液样品中的牛磺酸的含量,方法的加标回收率在93%~95%之间。更多还原

【Abstract】 A new method for the detection of taurine was developed using capillary electrophoresis by deriva-tization of 2,3-naphthalene-dicarboxaldehyde(NDA) pre-column.The effects of the derivatization time,detec-ted potential,running buffer pH,running buffer concentration and separation voltage were studied.Under the optimum conditions of detection,the linear range of taurine was 5.00×10-6～1.00×10-3mol/L.The corre-lation coefficient of the method was 0.9997.The concentration limit of detection LODc(S/N=3) was 1.60×10-6mol/L.The method was used to detected taurine both in normal hemolysis and abnormal hemolysis.The recovery of the method was between 93%～95%.更多还原