【摘要】 用离子交换层析和分子筛层析分离纯化了真叶期柠条根、速生期柠条叶、茎中的苯丙氨酸解氨酶(PAL),并测定了PAL全酶的分子量。用SDS-PAGE鉴定了全酶的组成,测定了各亚基的分子量。结果表明:柠条叶中存在306KD和222KD两种PAL,前者是由82KD和74KD亚基组成的异聚体,后者是由74KD亚基组成的同聚体;而柠条根和茎中仅存在74KD亚基组成的222KD全酶。该研究为pal基因的克隆及应用奠定了基础。更多还原

【Abstract】 Phenylalanine ammonia-lyase(PAL;EC 4.3.1.5) of root in leaf stage,leaf and branch in rapid growth stage of Caragana korshinskii,was purified by ion exchange chromatography and gel filtration chromatography.The subunits of PAL by SDS-PAGE were analyzed.The results showed that when purified by ion exchange chromatography and gel filtration chromatography,there were two kinds of PAL,one MW was 306KD and the other was 222KD,from leaf and one PAL which MW was 222 KD from stem and root.By SDS-PAGE,it could conclude that the PAL,MW 306KD,was composed of two polypeptides which MW might be 82KD and two polypeptides which MW might 74 KD,and the 222KD-MW PAL was composed of four homology subunits,which MW was 74KD.更多还原