【摘要】 以峨眉拟单性木兰带芽茎段为外植体,MS、WPM、B5为基本培养基,添加不同浓度的细胞分裂素(6-BA)和生长素(IBA、NAA、2,4-D),以不同浓度组合进行腋芽和愈伤组织的诱导。结果表明,基本培养基的类型是腋芽诱导的主要影响因素,以高盐浓度的培养基B5效果较好,MS次之,细胞分裂素的浓度与生长素的比值为15∶1时较适合腋芽的诱导,比值调整为5∶1时有利于茎的伸长生长;愈伤组织的诱导中6-BA起主导作用,其次是2,4-D,再者是基本培养基和NAA,其中2,4-D浓度过高不利于愈伤组织的诱导。更多还原

【Abstract】 Take the stem segment of Parakmeria omeiensis as explant,MS、WPM、B5 as culture medium with different density of 6-BA、IBA、NAA、2,4-D for axillary bud and callus induction.The results show that the type of the basic mediums are the main factor,medium of high salt concentration favored development of bud,B5 is the best,and then MS.The ratio of the cytokinin to auxins was adapted to the axillary bud induction 15∶1 and 5∶1 was adapted to the elongation growth of stem.6-BA is the leading role in callus induction,the second is 2,4-D,then the basic mediums and NAA,but in the higher concentration of 2,4-D could inhibit callus induction.更多还原