【摘要】 针对北海道黄杨(Euonymus japonicusThunb)组织培养过程中内生菌污染严重问题,使用山农一号进行杀菌和抑菌实验,结果表明:山农一号Ⅰ型能够有效对外殖体进行杀菌和抑菌,山农一号Ⅱ型添加在培养基中能有效抑制北海道黄杨组织培养过程中的内生菌.其中北海道黄杨茎段经山农一号Ⅰ型消毒后接种到含山农一号Ⅱ型的培养基中,杀菌、抑菌效果最佳,并且山农一号Ⅰ型和Ⅱ型对不定芽分化和外殖体的生长无抑制作用.更多还原

【Abstract】 In order to prevent and control the severe pollution in tissue culture of Euonymus japonicus Thunb,Shannong No.1 was applied to conducted the sterilization effect.The results showed that Shannong No.1 typeⅠcould sterilize and eliminate endophytic bacteria effectivly,Shannong No.1 typeⅡadded in the medium to effectivly inhibit endophytic bacteria in the tissue culture of Euonymus japnicus Thunb.The stem segments of Euonymus japonicus Thunb.used Shannong No.1 typeⅠdisinfection after inoculation into contain Shannong No.1 typeⅡin the culture medium,the effect of inhibitory and sterilization was optimum,and Shannong No.1 typeⅠand Ⅱthere be no inhibitory to the bud differentiation and growth of explant.更多还原