【摘要】 目的探讨p16INK4A蛋白在子宫颈鳞癌(SCC)和子宫颈上皮内肿瘤(CIN)中的表达及其与HPV16/18感染的关系。方法用原位杂交法检测HPV16/18在25例子宫颈癌、45例CIN及10例慢性子宫颈炎中的表达,同时用免疫组化EliVision法检测p16INK4A蛋白的表达。结果(1)与慢性子宫颈炎相比,CINⅡ级、CINⅢ级、浸润癌HPV16/18杂交信号阳性率显著增高(P<0.01);(2)子宫颈鳞癌组织、CINⅠ级、CINⅡ级、Ⅲ级及慢性子宫颈炎标本中p16INK4A蛋白阳性率分别为100.0%、20.0%、46.7%、100.0%和10.0%;(3)在子宫颈鳞癌及CINHPV16/18感染的标本中p16INK4A蛋白表达均是阳性。结论子宫颈鳞癌的形成与HPV感染、p16INK4A蛋白过表达是呈正相关关系,p16INK4A蛋白可能作为子宫颈鳞癌及CIN的标志物,对子宫颈癌筛查和预防有重要意义。更多还原

【Abstract】 Propose To investigate the relationship between the expression of p16INK4A in cervical squamous cell carcinomas (SCC) and cervical intraepithelial neoplasia (CIN) and HPV16/18 infection. Methods In situhybridization (ISH) were used to detect the expression of HPV16/18 in 25 cases of SCC, 45 cases of CIN and 10 cases of chronic cervicitis. And the expression of p16INK4A were detected by the immunohistochemical EliVision method. Results (1) The positive rate of HPV hybrid signals in CIN Ⅱ, CIN Ⅲ and SCC were all significantly higher than in chronic cervicitis ( P<0.01). (2) Positive expression rates of p16INK4A protein in cervical squamous carcinoma,CIN Ⅰ, CIN Ⅱ,CIN Ⅲ and chronic cervicitis were 100.0%,20.0%,46.7%,100.0% and 10.0%. (3)p16INK4A protein positive was found in which HPV16/18 infection of cervical squamous carcinoma and CIN. Conclusions There showed positive correlation between very high expression of p16INK4A protein and HPV infection in CIN and SCC.p16INK4A protein can be an indicator in SCC and CIN, Which has very significance in preventing the HPV infection and can work efficiently in SCC.更多还原