【摘要】 目的:探讨地塞米松对瘢痕疙瘩成纤维细胞基质金属蛋白酶1(又被称为间质胶原酶1)蛋白的分泌合成和mRNA表达的影响。方法:采用酶联免疫吸附实验(ELISA)和Northern杂交技术检测了培养瘢痕疙瘩成纤维细胞基质金属蛋白酶1蛋白的合成分泌和mRNA的表达。结果:培养瘢痕疙瘩成纤维细胞对照组基质金属蛋白酶1蛋白的浓度为(151.02±27.70)pg/mL;加入10-9M和10-6M含地塞米松培养基24小时后,基质金属蛋白酶的浓度分别为(121.27±45.57)pg/mL(P<0.05)和(101.78±35.82)pg/mL(P<0.01)。加入地塞米松24小时后,培养瘢痕疙瘩成纤维细胞的基质金属蛋白酶1mRNA的表达被显著抑制,经密度扫描定量分析:基质金属蛋白酶1 mRNA的表达分别下降了大约36%和53%。结论:地塞米松可抑制培养瘢痕疙瘩成纤维细胞基质金属蛋白酶1蛋白的合成分泌及其mRNA的表达,可能是地塞米松在抗瘢痕疙瘩纤维化作用中,尤其是纤维化形成后,疗效欠佳的原因。更多还原

【Abstract】 Objective:To investigate the effects of dexamethasone on the protein production and mRNA expression of matrix metalloproteinase 1(interstitial collagenase 1) in keloid fibroblasts. Methods:Matrix metalloproteinase 1 protein was measured by enzyme-linked immunosorbent assay(ELISA).The expression of matrix metalloproteinase 1 mRNA was determined by Northern hybridization.Results:The concentration of matrix metalloproteinase 1 protein was 151.02±27.70 pg/ml in control and changed to 121.27±45.57 pg/ml(P<0.05) and 101.78±35.82 pg/ml(P<0.01) after adding dexamethasone at concentrations of 10-9M and 10-6M respectively.The mRNA expression of matrix metalloproteinase 1 was markedly suppressed 24 hours after dexamethasone was added——the mRNA relative amount of matrix metalloproteinase decreased about 36% and 53% respectively.Conclusion:Dexamethasone exerted inhibitory effects on matrix metalloproteinase 1 at both the protein and mRNA levels.This function might related to the underlying mechanism that the anti-fibrosis effect is not effective enough especially after the development of fibrosis.更多还原