【摘要】 试验利用牛Y染色体重复序列作为雄性特异性引物,以肿瘤坏死因子(TNFα)为内标引物建立多重PCR和多重巢式PCR体系,进行牛早期胚胎性别鉴定。共设计4对引物—Y染色体重复序列外引物和内引物,其扩增片段大小分别为534bp和480bp,肿瘤坏死因子外引物和内引物,扩增片段大小分别为357bp和272bp。结果表明,4对引物均有很高的特异性和稳定性;多重PCR体系灵敏度为50pg(约8个细胞),多重巢式PCR体系灵敏度为10pg(约2个细胞),故多重巢式PCR体系更适合于牛胚胎性别鉴定。更多还原

【Abstract】 In this study,we designed four pairs of primers which the amplifiment products were 534bp,480bp,357bp and 272bp respectively length according to Y chromosome repeated sequence and tumor necrosis factor alpha(TNFa)for Sex Determination of Bovine Embryo.The result shows that these four pairs of primers all have highly specialty and stability.The nested PCR need only 10pg bovine genomic DNA to determine the sex of embryo,but the normal PCR need 50pg,so the nested PCR is more suitable for sex determination of bovine embryo.更多还原