【摘要】 通过D-113、110、D-151、NKC-9、001×7、D-072、D-062离子交换树脂对长春花中文多灵、长春质碱和长春碱的静态吸附容量、吸附率和解吸率等指标的考察,筛选出最佳树脂为D-151。对文多灵、长春质碱和长春碱的吸附容量分别为118.2mg/mL、102.2mg/mL和2.6mg/mL,并进一步考察其富集长春花3种生物碱的吸附和解吸附性能,确定了最佳的工艺条件和参数。吸附:上样溶液浓度为0.41mg生药/mL,pH为6.0,流速为3mL/min;解吸附:洗脱剂为20%乙醇除杂,90%乙醇(含2%氨水)洗脱。结果表明,经D-151离子交换树脂富集的长春花总生物碱中文多灵、长春质碱和长春碱的含量分别提高到34.53%、22.31%和5.86%,比传统溶剂萃取方法分别提高了2.34、3.49和2.07倍,回收率均在90%以上,D-151离子交换树脂可以用来富集长春花中的生物碱成分。更多还原

【Abstract】 The process parameters and conditions of the preconcentration of Vindoline, Catharanthine and Vinblastine with ion exchange resin were established. Seven different ion exchange resins named D-113, 110, D-151, NKC-9, 001×7, D-072 and D-062were studied on the static adsorption capacity, adsorption ratio and desorption ratio of Vindolin, Catharanthine and Vinblastine. The best resin was D-151 and the adsorption capacities of Vindoline, Catharanthine and Vinblastine were 118.2, 102.2, 2.6mg/mL, respectively. The optimum parameters were established by the study on adsorption and desorption characters of the three alkaloids with D-151. The parameters as follows, adsorption: sample concentration 0.41mg/mL, pH=6, flow rate 3mL/min; desorption: elution solvent 90% (including 2% ammonia)alcohol after 20% alcohol for removing polar components. The results showed that after treated with D-151, the concentrations of Vindoline, Catharanthine and Vinblastine were increased to 34.53%, 22.31% and 5.86% with 2.34, 3.49 and 2.07 times as high as traditional liquid-liquid extraction, respectively, and the yields of the three alkaloids were all above 90%. D-151 could be used to preconcentrate Vindoline, Catharanthine and Vinblastine.更多还原