【摘要】 目的:检测尿激酶纤溶酶原激活物(uPA)、尿激酶纤溶酶原激活物抑制剂(PAI-1)、血管内皮生长因子(VEGF)和微血管密度(MVD)在非小细胞肺癌(NSCLC)组织中的表达以及与淋巴结转移的关系。方法:采用免疫组织化学方法联合检测52例NSCLC组织中uPA、PAI-1、VEGF和MVD的表达水平。结果:uPA、PAI-1、VEGF和MVD在NSCLC中的表达显著高于正常肺组织,影响淋巴结转移的相关因素是TNM分期(P<0.001)、肿瘤侵犯程度(P=0.034)、uPA表达(P=0.048)、VEGF表达(P=0.047)。多因素分析表明VEGF高表达是淋巴结转移的独立影响因素(P=0.043)。结论:uPA、VEGF高表达与NSCLC的淋巴结转移密切相关,促进了肿瘤转移。更多还原

【Abstract】 Objective:Lymph nodes metastasis have focused on the relation between degradation of extracellular matrix,invasaion,migration,and tumor angiogenesis. The aim of this study is to evaluate the effects of the urokinase-type plasminogen activato(uPA) system and vascular endothelial cell growth factor(VEGF) on tumor invasion and lymph nodes metastasis in NSCLC. Methods:Expression of uPA,plasminogen activator inhibitor(PAI-1),VEGF and microvessel density(MVD) was retrospectively determined by ABC immunohistochemical technique in 52 patients with NSCLC. Results:The positive rates of uPA(80.8%),PAI-1(80.8%) and VEGF(75.0%) in NSCLC were respectively significantly higher than that in normal lung tisssues(P<0.001). A significant correlation of lymph nodes metastasis was found with uPA(P<0.048),VEGF(P<0.047),clinical stage(P<0.001) and tumor invasion(P<0.034),and multivariate regression analysis showed VEGF was the most risk factor. Conclusion:Overexpression of uPA and VEGF may contribute to lymph nodes metastasis.更多还原