【摘要】 目的探讨银杏叶提取物(EGb)对正常状态和冷束缚应激引起的胃肠功能紊乱的情况下大鼠胃动力的影响及可能机制。方法采用冷束缚应激的方法建立胃肠功能紊乱模型,应用葡聚糖蓝2000(DB-2000)作为染料观察EGb在正常及应激状态对胃排空速率的影响;并利用比色法观察大鼠胃组织和血浆中一氧化氮(nitricoxide,NO)含量的变化。结果在正常状态下,EGb对胃排空速率无显著性影响。冷束缚应激可导致大鼠胃排空减慢(P<0.05)。EGb干预可明显拮抗应激引起的胃排空减慢(P<0.05)。EGb对正常大鼠胃组织和血浆中NO含量没有影响;冷束缚应激可导致胃内NO含量降低(P<0.01),而血浆中NO升高(P<0.01)。EGb可降低应激引起的血浆NO含量的升高(P<0.01)。结论EGb在不同状态下对大鼠胃动力的影响有所不同,具有正相调节作用。NO可能参与了EGb对大鼠胃动力的调节作用。更多还原

【Abstract】 Objective To investage the effect of extract of ginkgo biloba(EGb) on gastric motility in different states and discuss the mechanism.Methods Using blue dextran 2000(DB 2000) as marker,we observed the effect of EGb on gastric emptying rate.And the content of NO in bllod plasma and stomach muscle layer were measured by chemical method. Results EGb had no effect on gastric motility in normal rat.cold-restraint stress inhibited significantly gastric emptying(P<0.05),and EGb attenated significantly stress-induced gastric emptying rate decrease(P<0.05).In the stress model group,NO content in blood plasma decrease markedly by EGb(P<0.01).Conclusion EGb can mediate gastric discord by cold restraint stress,NO might take part in the effect.更多还原