【摘要】 目的:探讨瘦素对人脐静脉内皮细胞(ECV-304)表达白细胞介素-8(IL-8)的影响。方法:用不同浓度的瘦素刺激ECV-304,检测ECV-304表达IL-8的情况。结果:在相同作用时间下,随着瘦素浓度的升高,IL-8蛋白及IL-8的mRNA的表达也随之升高;在相同瘦素浓度下,随着作用时间的延长,IL-8蛋白及IL-8的mRNA的表达也随之升高。结论:瘦素可以刺激ECV-304表达IL-8,而且呈时间和剂量相关性。更多还原

【Abstract】 Objective: To investigate the effect of leptin on the expression of IL-8 induced by ECV-304.Method:We used the leptin with different concentration to stimulate the primary culture ECV-304.Then the expression of IL-8 induced by ECV-304 was detected.Result:In the same action time,the expression of IL-8 protein and IL-8 mRNA improved when the level of leptin improved;meanwhile,in the same concentration of leptin,the expression of IL-8 protein and IL-8 mRNA improved when the action time extended.Both were significant when they were analyzed by the statistics.Conclusion:The expression of IL-8 induced by ECV-304 is correlated positively with the level of leptin and it′s action time.更多还原